Abstract
Background/purpose: Niche for osteogenesis forms near specialized blood vessels that highly express both CD31 and endomucin (Emcn). However, localization of the vessels remains unknown during wound healing process after pulp revascularization. We investigated the localization of both CD31 and Emcn-expressing vessels and osterix (Osx)-expressing cells after pulp revascularization. Materials and methods: Five-week-old male C57BL/6J mice underwent pulp revascularization in the mesial root canal of the right first maxillary molar. The mice were sacrificed at 1 hour, 7 days, 14 days, and 21 days post-surgery, and immunofluorescence for CD31, Emcn, and Osx was performed. Additionally, calcein labeling was used to visualize newly formed mineralized tissue. Results: At 1 hour post-surgery, blood clots were present, but no blood vessels or pulp tissues were observed. By day 7, the blood clots had disappeared, and CD31(+)/Emcn(+) blood vessels originating from the periapical tissue were found beneath the apical half of the root canal walls, alongside newly formed hard tissue and Osx(+) cells. By day 14, these blood vessels extended from the apex to the root canal orifice, and Osx(+) cells were observed nearby. Both CD31(+)/Emcn(+) vessels and Osx(+) cells, as well as mineralized tissue, significantly increased by day 14 (P
Recommended Citation
Ikarashi, Takatoshi; Mitomo, Keisuke; Tashiro, Kentaro; Haketa, Mayuka; Mizoguchi, Toshihide; Yamaguchi, Akira; and Muramatsu, Takashi
(2026)
"Blood vessels expressing CD31 and endomucin contribute to the formation of new hard tissue via osterix-expressing cells during the healing process of pulp revascularization in mice,"
Journal of Dental Sciences: Vol. 21:
Iss.
4, Article 19.
Available at:
https://jds.ads.org.tw/journal/vol21/iss4/19
Publication Date
2026
Received Date
Apr 17 2026
Accepted Date
June 19 2026
Final Revision Date
June 19 2026
