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DOI

10.1016/j.jds.2021.12.021

First Page

1221

Last Page

1228

Abstract

Abstract Background/purpose Previous studies have shown that lncRNA nuclear autosomal transcript 1 (NEAT1) is abnormally expressed in periodontitis patients. However, the pathological mechanism of NEAT1 regulating periodontitis is still not clear. This study attempted to explore the expression of NEAT1 in periodontitis patients and its effect on periodontitis cell model and inflammatory response. Materials and methods The expressions of NEAT1 and miR-205-5p in gingival crevicular fluid (GCF) and cell samples were analyzed by qRT-PCR. The diagnostic value of NEAT1 in periodontitis was evaluated by constructing an ROC curve. The efficacy of NEAT1 on cell function and inflammatory response were assessed in LPS-induced PDLSCs. Luciferase reporter gene assay verified the targeting relationship between miR-205-5p and NEAT1. Results In the clinical section of this study, it was observed that NEAT1 expression was increased in GCF of periodontitis patients, and NEAT1 was found to be useful for periodontitis diagnosis. Besides, in vitro experiments suggested that inhibition of NEAT1 could improve cell viability and attenuated cell apoptosis and generation of inflammatory factors. Subsequently, luciferase reporter gene assay revealed that miR-205-5p was the target gene of NEAT1, and was negatively regulated by NEAT1. Conclusion High expression of NEAT1 has diagnostic value for periodontitis, and NEAT1 knockdown may reduce LPS-induced cell damage by increasing the level of miR-205-5p, which may provide a new breakthrough for the diagnosis and treatment of periodontitis.

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