DOI
10.1016/j.jds.2023.01.020
First Page
1630
Last Page
1637
Abstract
Background/purpose Testing of dental materials when in contact with innate immune cells has been so far hindered by the lack of proper in vitro models. Human primary monocyte-derived macrophages (MDMs) would be an excellent option to this aim. However, the inability to detach them from the tissue culture plates contrast the possibility to culture them on biomaterials. The goal of the present work is to present and validate an innovative protocol to obtain MDMs from peripheral blood monocytes, and to reseed them in contact with biomaterials without altering their viability and phenotype. Materials and methods We differentiated MDMs on ultra-low attachment tissue culture plastics and recovered them with specific detachment solution in order to be reseeded on a secondary substrate. Therefore, using biological assays (RT-PCR, Western blot, and immunofluorescence) we compared their phenotype to MDMs differentiated on standard culture plates. Results Transferred MDMs keep their differentiated M0 resting state, as well as the ability to be polarized into M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages. Conclusion These data provide the dental material research community the unprecedented possibility to investigate the immunomodulatory properties of biomaterials for dental application.
Recommended Citation
Ghezzi, Benedetta; Bianchi, Massimiliano G.; Ghezzi, Benedetta; Maurizi, Eleonora; Macaluso, Guido M.; Bussolati, Ovidio; and Lumetti, Simone
(2023)
"Preparation of human primary macrophages to study the polarization from monocyte-derived macrophages to pro- or anti-inflammatory macrophages at biomaterial interface in vitro,"
Journal of Dental Sciences: Vol. 18:
Iss.
4, Article 65.
DOI: 10.1016/j.jds.2023.01.020
Available at:
https://jds.ads.org.tw/journal/vol18/iss4/65
