DOI
10.1016/j.jds.2022.08.034
First Page
893
Last Page
897
Abstract
Abstract Three-dimensional (3D) cell culture systems are reported to be more physiologically similar to the in vivo state than 2-dimensional (2D) models, which are extensively employed in periodontal research. Herein, we developed a 3D gingival tissue model with both epithelial and lamina propria layers using human gingival epithelial Ca9-22 cells and primary gingival fibroblasts. The epithelial layer of the developed 3D gingival tissue culture was treated with butyrate, a metabolite of oral bacteria, and the treatment induced the release of damage-associated molecular patterns, such as DNA and Sin3A associated protein 130 kDa (SAP130). Taken together, butyrate exposure to the epithelium of 3D gingival epithelial-connective tissue hybrid systems could induce epithelial cell death and the subsequent release of damage-associated molecular patterns.
Recommended Citation
Tsuda, Hiromasa; Kurosawa, Yusuke; Yamaguchi, Hirofumi; Uemichi, Kazuki; Shinozuka, Keiji; and Kirihara, Yuki
(2023)
"Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture system,"
Journal of Dental Sciences: Vol. 18:
Iss.
2, Article 70.
DOI: 10.1016/j.jds.2022.08.034
Available at:
https://jds.ads.org.tw/journal/vol18/iss2/70
