•  
  •  
 

DOI

10.1016/j.jds.2019.09.006

First Page

65

Last Page

74

Abstract

Abstract Background/purpose Enterococcus faecalis ( E. faecalis ) is considered a predominant pathogen for persistent periapical infections. Antisense walR (AS walR ) RNA was reported to inhibit the biofilm formation and sensitized E. faecalis to calcium hydroxide medication. The aims of this study were to investigate whether the graphene oxide (GO) nanosheets could be used to enhance antibacterial activity of AS walR RNA for E. faecalis in periapical periodontitis. Materials and methods We developed a graphene-based plasmid transformation system by loading antisense walR plasmid with GO-polyethylenimine (PEI) complexes (GO-PEI-AS walR ). The particle size distributions and zeta-potential of the GO-PEI-AS walR were evaluated. Then, AS walR plasmids were labeled with gene encoding enhanced green fluorescent protein (AS wal R-eGFP). The transformation efficiencies and the bacterial viability of E. faecalis were evaluated by confocal laser scanning microscopy. Quantitative real-time PCR assays were used to investigate the expressions of E. faecalis virulent genes after transformed by GO-PEI-AS walR . Also, the antibacterial properties of the GO-PEI-AS walR were validated in the rat periapical periodontitis model. Results We showed that GO-PEI could efficiently deliver the AS walR plasmid into E. faecalis cell. GO-PEI-AS walR significantly reduced virulent-associated gene expressions. Furthermore, GO-PEI-AS walR suppressed biofilm aggregation and improved bactericidal effects using infected canal models in vitro . In four-weeks periapical infective rat models, the GO-PEI-AS walR strains remarkably reduced the periapical lesion size. Conclusion Transformation efficiency and antibacterial prosperity of AS walR can be marked improved by GO-PEI based delivery system for E. faecalis infections.

Download

Share

COinS